Survivin蛋白及其mRNA在直肠癌中的表达及意义

Correlation between the expression of Survivin and Invasion,Metastasis of human rectal cancer

目的:研究凋亡抑制因子Survivin蛋白及其mRNA在直肠癌组织中的表达及其与临床病理关系。方法:采用免疫组织化学方法检测40例直肠癌组织和20例正常组织中Survivin蛋白的表达量,采用实时荧光定量PCR方法检测25例直肠癌组织和19例正常组织中Survivin mRNA的表达量。结果:Survivin蛋白在直肠癌组织中的阳性表达率为75%(30/40),在正常直肠组织中的阳性表达率为0%(0/20),survivin mRNA在直肠癌组织中的阳性表达率为68%(17/25),在正常组织中的阳性表达率为5%(1/20)。在癌组织中,Survivin蛋白及其mR-NA的表达量与患者的性别分期和年龄无关,与组织的分化程度、Dukes'和有无淋巴结转移密切相关。结论:Survivin可能在直肠癌的发生中发挥重要作用,可以作为一种肿瘤标记物用于直肠癌的预后。

Objective： To explore the expression of survivin protein and its mRNA in rectal cancer and their clinic significance. Methods：Immunehistochemistry was used to detect the expression of survivin protein in the 40 rectal cancer tissues and 20 normal colorectal tissues ; SYBR Green Ⅰ Real - time fluorescence quantitative PCR were used to detect the expression of survivin mRNA in 25 rectal cancer tissues and 20 normal rectal tissues. Results： the positive of survivin protein expression in rectal cancer tissues is 75% （30/40）, but in normal rectal tissues is 0% （0/20） ; survivin mRNA expression in rectal cancer tissues is 68% （17/25）, hut in normal rectal tissues is 5% （1/20） ; In cancer tissues, survivin protein and its mRNA expression has correlation with patients＇histological differentiation, Dukes＇clinic stage and lymph node metastasis. Conclusions ： Survivin may play an important role in the genesis of rectal cancer and can be seen as a tumor marker in the prognosis of rectal cancer.