RNA干扰沉默Livin基因表达对食管癌裸鼠移植瘤的抑制作用

The Inhibition Effect of RNA Interference Silence Livin Gene Expression in Transplantation Tumor of Esophageal Carcinoma in Athymic Mouse

目的利用RNA干扰技术在裸鼠体内抑制Livin基因表达,探讨RNA干扰对食管癌裸鼠移植瘤生长的抑制作用。方法设计靶向Livin基因的siRNA,构建重组siRNA表达载体pRNAT-U6.1-siLivin并导入裸鼠皮下移植瘤,观察肿瘤生长情况。采用RT-PCR方法检测干预组和对照组Livin基因表达。结果pRNAT-U6.1-siLivin导入裸鼠皮下移植瘤后14 d,肿瘤体积明显变小。LivinmRNA表达明显降低。结论pRNAT-U6.1-siLivin可以明显抑制食管癌裸鼠移植瘤的增殖。

Objective To inhibit the Livin gene expression in athymic mouse by RNA interference and to observe the inhibition effect in transplantation tumor of esophageal carcinoma in athymic mouse. Methods To design and construct the pRNAT-U6. 1-siLivin expression vectors, then to inject them into the athymie mouse with transplantation tumor and to observe its growth. To detect the Livin gene expression between the experimental group and the control group by RT-PCR. Results The gross tumor volume became small obviously as well as the expression of Livin mRNA lowered after 14 days of the pRNAT-U6.1-siLivin injected into transplantation tumor of athymie mouse. Conclusion The pRNAT-U6, 1-siLivin can obviously inhibit the proliferation of transplantation tumor of esophageal carcinoma in athymic mouse.