黄芪复方制剂对重症肌无力患者淋巴细胞亚群的影响

Effect of compound preparation of Astragalus mongholicus on subsets of lymphocytes in patients with myasthenia gravis

目的探讨黄芪复方制剂对重症肌无力(MG)患者的细胞免疫调节机制。方法将60例患者随机分为治疗组30例、对照组30例,治疗组服用黄芪复方制剂;对照组服用泼尼松片,疗程12周。应用流式细胞仪检测重症肌无力患者治疗前后外周血淋巴细胞亚群分布的变化情况。结果两组患者治疗后CD4+细胞百分率、CD4+/CD8+比值均有明显下降,治疗组CD4+/CD8+比值与对照组比较差异有统计学意义(P<0.05)。治疗组治疗后CD8+细胞百分率明显增加,与治疗前比较差异有统计学意义(P<0.05),与对照组比较无明显变化(P>0.05)。结论通过调节淋巴细胞亚群比例分布以及体液免疫功能等的变化,可能是黄芪复方制剂发挥免疫调节作用机制之一。

Objective To investigate the effect of compound preparation of Astragalus mongholicus on subsets of lymphocytes in patients with myasthenia gravis （MG）, and to explore its regulatory mechanism. Methods 60 MG patients were randomly divided into test group （n=30, given compound preparation of Astragalus mongholicus for 12 wks） and control group （n=30, given prednisone tablets for 12 wks）. Flow eytometry was applied to examining the distribution change of the subsets of lymphocytes in patients before and after treatment. Results After treatment, the percentages of CD4^＋ T cells and the ratio of CD4^＋/CD8^＋ in both groups decreased compared with those before treatment. The ratio of CD4^＋ /CD8^＋ in test group was significantly lower than that in control group （P〈0.05）. The percentage of CD8^＋ T cells in test group after treatment increased significantly （P〈0.05） compared with that before treatment, but had no significant difference compared with that in control group （P〉0.05）. Conclusion The result from the current study suggests that one of the mechanisms of the compound preparation of Astragalus mongholicus regulating immune response may be achieved through its modulating effect on the distribution of subsets of lymphocytes and humoral immune function.