可酶降解的温度及pH敏感水凝胶的制备及对牛血清白蛋白的吸附和释放

Synthesis,Absorption and Release of BSA of Enzymatically Degradable pH- and Temperature-sensitive Hydrogels

以4,4′-二甲基丙烯酰氨基偶氮苯(BMAAB)为偶氮交联剂,制备了可酶降解的N-异丙基丙烯酰胺(NIPAAm)-丙烯酸(AA)共聚物水凝胶。结果表明,研究的凝胶均表现出在4℃和37℃之间的溶胀相转变。以牛血清白蛋白(BSA)为模型药物,在pH=7.4的缓冲溶液中对BSA进行了负载。结果表明,凝胶在4℃时对BSA的负载量为144.5 mg(BSA)/g(gel),37℃为14.8 mg(BSA)/g(gel)。凝胶在pH=7.4的缓冲溶液中,在结肠菌作用下能发生酶降解,药物累积释放量4 d可达100 mg(BSA)/g干胶。

The enzymatically degradable poly（N-isopropylacrylamide-co-acrylic acid） hydrogels were prepared with 4,4′-bis（methacryloylamino）azobenzene（BMAAB） as a crosslinker.It was found that the hydrogels exhibited a phase transition temperature between 4 ℃（refrigerator temperature） and 37 ℃（human body temperature）.Bovine Serum albumin（BSA） as a model drug was loaded into the hydrogels.The loading capability was 144.5 mg BSA/g gel in buffer solution（pH=7.4） at 4 ℃,while it was 14.8 mg BSA/g gel at 37 ℃.The hydrogels can be degraded by cecal bacteria and cumulative release may be 100 mg BSA/g gel 4 days after enzymatic degradation of hydrogel in pH=7.4 buffer solution containing cecal bacteria at 37 ℃.