人骨髓间充质干细胞对再生障碍性贫血患者T细胞活化的影响

Effect of human bone marrow mesenchymal stem cell on T cell activation in patients with aplastic anemia

目的探讨人骨髓间充质千细胞在体外对再生障碍性贫血患者T细胞活化的影响。方法从人骨髓分离培养间充质干细胞，用尼龙棉柱分离T淋巴细胞，分别以不同数量间充质干细胞加入到植物血凝素刺激的再生障碍性贫血患者T淋巴细胞活化体系中，通过流式细胞仪获取数据，计算CD3^＋CD25^＋和CD3^＋CD38^＋T细胞的表达率。结果当间充质干细胞为1×10^4／孔时，与单独培养的再生障碍性贫血患者T淋巴细胞（对照组）相比，间充质干细胞对再生障碍性贫血患者T淋巴细胞CD25及CD38的表达呈明显抑制作用，差异有统计学意义（P〈0．01）。当间充质干细胞为1×10^3／孔时，与对照组相比无统计学意义。结论间充质干细胞在体外抑制再生障碍性贫血患者T细胞的活化，且这种抑制作用具有数量依赖性。

Objective To observe effect of human bone marrow mesenchymal stern cell（MSC） on T cell activation in patients with aplastic anemia（AA）. Methods MSCs were separated with Percoll and cultured in low glucose DMEM. T cells were harvested by using nylon column. MSCs of various concentrations were added to PHA induction T cell activation cultures. The CD25 and CD38 expression rates of CD3^＋ T cells was analyzed by flow cytometry. Results MSCs were planted in 96 well plates （ 1 × 10^4/well for group A） and cocuhured with T cell isolated from peripheral blood of AA patients. Peripheral blood T cell noncocuhured with MSC acted as the control group, MSCs inhibit PHA induced the CD25 and CD38 expression of CD3 ^＋ T cells in patients with AA. The expression rates were significantly lower than control group （ P 〈 0. 01 ）. But the expression rates showed no significant difference between the two groups （MSCs , 1 × 10^3/well for group B）. Conclusion Human bone marrow MSCs inhibit T cell activation in patients with AA in vitro and the inhibitory effects are dependent on the amount of MSCs.