不同浓度锈菌对大豆叶片的致病反应

Pathogenic effects following different concentrations of Phakopsora pachyrhizi suspension on soybean leaves

采用离体叶片接种法,观察不同浓度夏孢子悬浮液对大豆叶片的致病反应。结果表明:各个接种浓度均可引起叶片发病,发病率随接种浓度的升高而增加,夏孢子堆密度随接种浓度升高而增加,夏孢子堆破裂时间随接种浓度的增加而缩短,不同品种的致病反应差异主要表现在病斑出现时间、夏孢子堆密度、夏孢子堆破裂时间和发病率等方面。锈菌浓度对孢子堆大小没有显著影响,采用105个夏孢子/mL的接种浓度可以有效地区分不同品种的抗病性。

The detached leaf inoculation method was used to study the pathogenic effect caused by different concentration of suspension of Phakopsora pachyrhizi on soybean varieties with different rust resistance. The results showed that each concentration treatment could result in the pathogen infection. Significant differences in disease incidence, the time for disease spot appearance, uredia density and the erumpent time for uredia were found among the varieties. The disease incidence, uredia density increased with the increase of suspension concentration. The time for disease spot appearance, and the erumpent time for uredia decreased with the increase of suspension concentration. The 10^5spores/mL suspension was optimal to distinguish the rust resistance of varieties.