寒地粳稻蛋白质含量在DH群体中的遗传变异研究

Study on hereditary variation of protein content of doubled haploid japonica rice in cold region

研究应用蛋白质含量(Protein Content,PC)不同的10个亲本,采用F1花药培养技术构建了寒地水稻6个组合的DH(Doubled Haploid,DH)群体。应用Choo和Reibergs提出的模型及公式,分析了这6个DH群体PC的分布、测算了遗传力、超亲率,检测了PC的基因对数及基因互作方式。结果表明,PC的遗传变异分为两大类型:5个组合PC的遗传是属于主效基因加微效多基因混合遗传,无主效基因互作;1个组合(松9798/龙花96-1530)PC的遗传属于多个主效基因加微效多基因混合遗传,存在主效基因间的互作,为互补作用,为PC的另一种遗传模式,且是产生PC超亲现象的主要原因之一。PC在DH群体的遗传力为0.77,PC的超低亲率较低,控制PC的基因对数为3.2￣6.5对,平均4.8对。

The DH population of 6 combinations had been constructed in a cold rice region by technology of antheral culture in hybridization combination F1 which is from 10 parents of different protein contents and analyzed hereditary variation distribution of protein contents in DH population, estimated hereditary capacity and rate over parent in DH population by the model and formula which were presented by Choo and Reibergs, and examined the number of gene pairs and the pattern of gene interaction which controlled these characters. In this study, hereditary variation of protein content was divided into two types： type Ⅰ was heredity of protein content in 5 crosses which belonged to blending heredity which were controlled by major gene which had hereditary feature of quantitative character and many minor genes, there was no interaction of major genes. Type Ⅱ was heredity of protein content in only one cross （Song9798/Longhua96-1530） which belonged to blending heredity controlling many major genes that had hereditary feature of quantitative character and many minor genes, there was epistasis effect between major genes, which was over lapped effect. Hereditary capacity of protein content was 0.77 in DH population, and the rate over low parent was relatively low. The number of gene pairs was 3.2-6.5 pairs controlling protein content, 4.8 pairs on average.