三拗汤对人外周血来源的树突状细胞成熟的影响

Effect of on maturation of human peripheral blood monocyte derived dendritic cells

目的探讨三拗汤对人外周血单核细胞来源的树突状细胞分化成熟的影响。方法采用体外培养人外周血单核细胞来源的未成熟树突状细胞,将细胞随机分为模型组和用药组,用药组加入三拗汤水煎液+LPS;模型组加入相应体积的无菌双蒸水+LPS,在倒置相差显微镜下观察细胞的形态改变并计数。结果模型组成熟树突状细胞数量较多,细胞表面有许多毛刺状突起;而用药组未成熟树突状细胞较多,多数细胞仍为圆形,表面少有突起。两者比较有差异(P<0.05)。结论三拗汤可抑制树突状细胞的分化和成熟。

Objective To investigate the effect of Sanaotang （ composed of Ephedra sinica, Prunus armeniaca and Glycyrrhiza uralensis） on maturation of human peripheral blood monocyte derived dendritic cells （DC）. Methods DCs were isolated from peripheral blood mononuclear leukocytes harvested,further cultured in vitro,then into dendritic cells with 100 ng/ml GM-CSF and 20 ng/ml IL-4. DCs were divided into two groups, control group and Sanaotang-treatment group. The morphology characteristics and the number of DCs from the cultures were microscopic photoed. Results The induced DC had a couple of large and long processes on ceil surface, which were the typical morphological characteristics of DC in the control group. The number of mature DCs in the control group was more than that in the treatment group （P 〈 0. 05 ）. Compared with the control group, DCs cultured in the presence of Sanaotang displayed less dendritic pseudopod. Conclusion Sanaotang can inhibit the maturation and differentiation of dendritic cells from human neripheral blood.