蛋白激酶C抑制剂对CNE-2Z细胞周期的影响

EFFECT OF PROTEIN KINASE C INHIBITORS ON CNE-2Z CELL CYCLE

目的：蛋白激酶C（PKC）抑制剂诱导CNE－2Z细胞凋亡时细胞周期的观察。方法：PKC催化区抑制剂Staurospoine（ST）和调节区抑制剂Sphingosine（SS），终浓度分别为1X10-6mol／L和4X10-5mol／L，诱导CNE－2Z细胞24h，用流式细胞仪（FCM）分析。结果：诱导组细胞周期与对照组比较，ST使细胞G1和S期明显减少及G2期明显增加（P＜0．01）；SS使S期增加和G2期减少明显（P＜0．05），而G1期变化不大（P＞0．05）。诱导组G1期前均有明显的亚二倍体峰（凋亡峰）。结论：PKC抑制剂SS、ST诱导CNE－2Z细胞凋亡，同时存在明显的细胞周期异常改变。

Objective:To observe the effect of protein kinase C (PKC)on cell cycle during the induction of CNE-2Z apoptosis. Methods: Propidium iodide (PI ) staining and flow cytometry (FCM ) analysis were used. CNE-2Z cells were respectively cultured for 24 hours with Staurospoine (ST),an inhibitor acting on the catalytic domain,and Sphingosine (SS),an inhibitor acting on modulating domain,at the concentration of 1 X 10 -6mol/L and 4 X 10-5mol/L respectively. Results: Compared with the control group, the percentage of G1 and S cells was decreased and that of G2 cells was increased significantly in the ST treated group(P<0.01 ),and the percentage of S cells was increased and that of G2 cells was decreased obviously(P<0.05)but that of G1 cells didn't significantly change in the SS treated group. Hypodiploid DNA peaks (apoptotic peak) were seen in the treated groups. Conclusion: Protein kinase C inhibitors could induce the apoptosis of CNE-2Z cells with the distinctive change of cell cycle.