苏云金杆菌营养期杀虫蛋白Vip3A-LS1对2种林业害虫活性的研究

Bioassay of Bt Vip3A-LS1 Protein against Hyphantria cunea and Clostera anachoreta

以实验室克隆的苏云金芽孢杆菌Vip3A-LS1基因为材料,利用原核表达载体pET-21b,构建重组载体表达蛋白,并测定了表达蛋白对美国白蛾和杨扇舟蛾幼虫的活性。结果表明:表达重组质粒pEV113经IPTG诱导得到了约88kD的Vip3A-LS1蛋白;该蛋白对美国白蛾和杨扇舟蛾均有一定的杀虫活性,80μg蛋白对初孵美国白蛾平均校正死亡率达到(53.02±0.43)%,体质量抑制率达到了80.60%,对杨扇舟蛾初孵幼虫平均校正死亡率达到(68.00±0.60)%,抑制作用均达到80%以上。

Recombinant vector of pEV113, Vip3A-LS1 gene inserted to pET21-b vector, was constructed. The bioassay against Hyphantria cunea （Drury） and Clostera anachoreta was conducted. 88 kD protein of Vip3A-LS1 was obtained by SDS- PAGE. The bioassay showed that the protein was toxic to the 1 st instar larvae of H. cunea and C. artachoreta, and the corrected mortality rates for 80 p,g protein were （53.02±0.43）% and （68.00±0.60）% against H. cunea and C. anachoreta respectively. The weight inhibitory rates against 1st instar larvae of H. cunea were 80.60% and against C. anachoreta was over 80%.