COX-2、EGFR和Ki67在大肠癌组织中的表达及其意义

Expression of cyclooxygenase-2,epidermal growth factor receptor and Ki67 in colorectal cancer and its significance

目的探讨环氧合酶-2(COX-2)、表皮生长因子受体(EGFR)和Ki67在大肠癌组织中的表达及其意义。方法选取大肠癌70例、大肠腺瘤21例和正常大肠组织15例,采用免疫组织化学Envision方法检测COX-2、EGFR和Ki67的表达。结果在正常大肠黏膜、大肠腺瘤和大肠癌组织中,COX-2的阳性表达率分别为33.3%、71.4%和80.0%,大肠癌和腺瘤组显著高于正常组(P均<0.05);EGFR的阳性表达率依次为0、23.8%和65.7%,两两比较有统计学差异(P均<0.05);Ki67的阳性表达率依次为40.0%、76.2%和82.8%,大肠癌和腺瘤组织中的表达均高于正常大肠组织(P均<0.05)。COX-2在有淋巴结转移的大肠癌组中的表达明显高于无淋巴结转移组(P<0.05);EGFR在低分化、高分期和有淋巴结转移大肠癌中的表达明显增强(P均<0.05),且COX-2与EG-FR、COX-2与Ki67、EGFR与Ki67之间均具有相关性(r=0.316,0.435,0.314,P均<0.05)。结论COX-2、EGFR和Ki67对大肠癌的发生、发展有协同作用。

Objective To investigate the expression of COX-2, EGFR and Ki67 in colorectal cancer and its significance. Methods To select 70 samples of colorectal cancer,21 samples of colorectal adenomas and 15 samples of normal colorectal mueosal tissues,the expressions of COX-2, EGFR and Ki67 were evaluated by method of Envision. Results In normal colorectal mucosal tissues , colorectal adenomas and colorectal cancer, the positive rates of COX-2 expression were 33.3% ,71.4% and 80.0% ,respectively. In normal colorectal mucosal tissues ,eoloreetal adenomas and colorectal cancer, the positive expression rates of EGFR were 0,23.8% and 65.7% respectively, there were statistical differences ,when they compared with each other（ P 〈 0.05 ）. The positive expressions of Ki67 were 40.0% ,76.2% and 82.8%, Ki67 expressions in colorectal cancer group and adenomas group were significantly higher than that of the normal group（P 〈 0.05 ）. In colorectal cancer, the expression of COX-2 in the group with lymph nodes metastasis was significantly higher than the group with no lymph nodes metastasis （ P 〈 0.05 ）. The expression of EGFR in the group of poorly differentiation, high stage and lymph nodes metastasis was significantly higher than the group of the well differentiation, low stage and no lymph nodes metastasis in colorectal cancer （P 〈 0.05）. Furthermore, the studies showed that there were significant correlation between the expression of COX-2 and EGFR , COX-2 and Ki67 as well as EGFR and Ki67 （r = 0. 316, 0. 435,0.314,P all 〈 0.05）. Conclusions COX-2 , EGFR and Ki67ptay an important role in the carcinogenesis and development of colorectal cancer.