人工高眼压家兔血液、虹膜及房水cAMP、cGMP含量的关系

Quantitative analysis of cAMP and cGMP in the plasma, iris and aqueous humor in rabbits with high intraocular pressure

目的研究家兔血液、虹膜及房水中环腺苷酸（ｃｙｃｌｉｃａｄｅｎｏｓｉｎｅｍｏｎｏｐｈｏｓｐｈａｔｅ，ｃＡＭＰ）和环鸟苷酸（ｃｙｃｌｉｃｇｕａｎｏｓｉｎｅｍｏｎｏｐｈｏｓｐｈａｔｅ，ｃＧＭＰ）含量与高眼压之间的关系。方法用放射免疫方法测定家兔血浆、虹膜和房水中ｃＡＭＰ、ｃＧＭＰ含量。结果ｃＡＭＰ含量（ｎｍｏｌ／Ｌ）：血浆为７２．８１±７．６７，虹膜为１４１．０８±１６．７０，房水中为２８．４０±０．４９；ｃＧＭＰ含量（ｎｍｏｌ／Ｌ）：血浆为１０．３７±６．１５，虹膜为３５．６４±７．０２，房水中为１２．９０±０．６１。ｃＡＭＰ含量高于对照组（Ｐ＜０．０１），ｃＧＭＰ低于对照组（Ｐ＜０．０１）。结论高眼压与ｃＡＭＰ、ｃＧＭＰ含量关系显著。

Objective To study the relationship between the levels of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) in the plasma, iris and aqueous humor and high intraocular pressure in rabbits. Methods The quantitative tests of cAMP and cGMP from rabbits were carried out by radioimmunoassay technique. Results The results showed that there were 72.81±7.67, 141.08±16.70 and 28.40±0.49 of cAMP (nmol/L), and there were 10.37±6.15, 35.64±7.02 and 12.90±0.61 of cGMP (nmol/L) in the plasma, iris and aqueous humor respectively. The levels of cAMP were higher than those in the same samples from the animals as controls (P<0.01), while the levels of cGMP were less than controls (P<0.01). Conclusion High intraocular pressure may be significantly associated with the levels of cAMP and cGMP.