摘要
为观察p53基因和Rb基因与鼻咽癌发生的关系,应用聚合酶链反应-单链构型多态分析技术(PCR-SSCP技术),检测33例鼻咽癌组织、4例鼻咽部炎性组织及3例正常鼻咽部组织中p53基因第7,8外显子的突变。结果显示,在33例鼻咽癌中有7例存在p53基因第8外显子的突变,突变率为21.2%。在第7外显子中未检测出突变发生。应用Southern印迹杂交,对Rb基因在鼻咽癌中存在状态进行研究。结果表明,在13例鼻咽癌组织中有11例鼻咽癌组织有Rb基因的缺失或明显减弱,提示p53基因突变及Rb基因的缺失而导致的基因失活与鼻咽癌的发生有密切关系。
In order to observe the relation of tumor supperssor gene p53 and Rb gene to nasopharyngeal carcinoma, we investigated p53 gene mutations in exon 7 8 in 33 cases of nasopharygeal carcinoma (NPC), using single strand conformation ploymorphism analysis of PCR (PCR SSCP). The data showed p53 mutations in 7 of 33 specimens at exon 8(21.2%). No mutation in exon 7 was detected. Analysis of Rb gene in NPC tissues by southern blot revealed the deletion and loss of activity of Rb gene in 11 of 13 cases (86.6%). Our results suggest that Rb gene and p53 gene are closely associated with the tumorigenesis of NPC.
出处
《华西医科大学学报》
CSCD
1997年第3期268-271,共4页
Journal of West China University of Medical Sciences
关键词
P53基因
RB基因
鼻咽肿瘤
抑制基因
p53 gene Rb gene Nasopharyngeal carcinome Gene mutation Gene deletion