印楝异交率和基因流的分析

Outcrossing Rate and Gene Flow Estimation of Azadirachta indica

利用RAPD分子标记,对引种于云南省元谋县和红河县的印楝种群进行父本分析。用12个引物对4个种群共7株树体测定了异交率,平均异交率为96.27%,异交率95%置信区间为93.50%～99.05%。对其中红河县勐龙乡大黑公种群的2株母本E和F及周围96株父本进行了基因流测定,遗传排除法确定了各有2个父本向母本E和F传粉,最大似然法分析得出有3个父本向母本E传粉,有10个父本向母本F传粉。有52.9%的传粉集中在以母本为圆心,半径30～50m的范围内。来自主风方向和逆风方向的传粉在数量上无差异。

Random Amplified Polymorphic DNA was used to estimate the outcrossing rate and gene flow of Azadirachta indica introduced to Yuanmou County and Honghe County, Yunnan, China. Seven trees from four populations were chosen as the maternal parents, and each seedlings of each maternal tree were randomly selected as identified outcrossed offspring, characterized by DNA of their ripe seeds was collected. After germination, 23 sib code to estimate the outcrossing rate. Each primer fragments that maternal trees do not have. The cumulative outcrossing rate of each mother tree was calculated by a total of 12 primers. The average outcrossing rate of neem （Azadirachta indica A. Juss. ） was 96.27% , with a high 95% confidence interval from 93.50% through 99.05%. The Daheigong population was also used for a gene flow test among the 4 populations. Two mother trees, E and F, were maternal parents, and 96 trees around them were paternal parents. Two methods, genetic exclusion and maximum likelihood, were used to estimate gene flow of the population. With 12 primers, each of 2 paternal trees was confirmed to have dispersed pollen to E and F by genetic exclusion. And maximum likelihood results indicated that 3 paternal trees had distributed pollen to maternal tree E, while 10 paternal trees had distributed pollen to maternal tree F. According to the map, 52.9% of pollen dispersal was as far as 30--50 m, and the amount of downwind distribution was not significantly distinct from the upwind.