急性髓细胞白血病细胞IL-2受体基因的研究

A Study on Interleukin 2 Receptor Genes in Acute Myeloid Leukemia Cells

为研究髓细胞白血病（ＡＭＬ）细胞白介素－２受体（ＩＬ－２Ｒ）基因及ＩＬ－２在ＡＭＬ中的作用，用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）对４１例ＡＭＬ患者化疗前白血病细胞ＩＬ－２ＲαｍＲＮＡ和ＩＬ－２ＲβｍＲＮＡ进行检测，并对部分ＩＬ－２Ｒ基因阳性表达细胞用３Ｈ－ＴｄＲ掺入法测定其对重组人ＩＬ－２（ｒｈＩＬ－２）的反应。结果显示，ＡＭＬ细胞ＩＬ－２Ｒα和ＩＬ－２β基因表达是一普遍现象，４１例中白血病细胞有ＩＬ－２ＲαｍＲＮＡ和ＩＬ－２ＲβｍＲＮＡ表达分别为２８例（６８．３％）和３２例（７８．０％），ＩＬ－２ＲαｍＲＮＡ和ＩＬ－２ＲβｍＲＮＡ同时表达者２１例（５１．２％）。ＩＬ－２Ｒ基因阳性表达细胞对ｒｈＩＬ－２的体外反应呈不均一性，表现为细胞增殖、受抑或不反应，８例中仅１例Ｍ５型患者细胞呈增殖反应。本研究表明ＡＭＬ细胞ＩＬ－２Ｒ基因表达是一普遍现象，这些细胞在体外对ｒｈＩＬ－２的反应呈不均一性，大多表现为无反应或受抑。

To investigate IL 2 receptor (IL 2R) gene expression in acute myeloid leukemia (AML) cells and the role of IL 2 in AML, the IL 2Rα mRNA and IL 2Rβ mRNA in leukemic cells obtained from bone marrow of 41 cases with AML before chemothe rapy were measured by reverse transcriptase polymerase chain reaction (RT PCR). The responses to recombinatnt human IL 2 (rhIL 2) of these leukemic cells expressing IL 2R mRNA from 8 cases were assayed by 3H thymidine incorporation. The IL 2Rα mRNA and IL 2Rβ mRNAs in leukemic cells were detected in 28 cases (68.3%) and 32 cases (78.0%), respectively. The IL 2Rα mRNA and IL 2Rβ mRNA in leukemic cells of 21 cases (51.2%) were expressed simultaneously. The responses to rhIL 2 of these leukemic cells from 8 cases were heterogeneous. The responses to rhIL 2 appeared in three types: proliferation, inhibition and no reaction. Only the cells of one case with M5 expressing IL 2Rα mRNA and IL 2Rβ mRNA responded proliferatively. The results demonstrate that the expressions of IL 2R genes in AML cells are a general phenomenon. The responses of these leukemic cells to rhIL 2 in vitro are heterogeneous.