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分光光度法检测恶性肿瘤细胞ATP含量及化疗药物对其影响的初步研究 被引量:3

Application of Spectrophotometry to Evaluation of the Levels and the Influence of Chemotherapeutic Drugs on ATP of Malignant Cell Lines
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摘要 为探讨ATP分光光度法应用于恶性肿瘤体外化学药物敏试的价值,作者将该法用于Hela和L929恶性肿瘤细胞系的检测。结果显示:当实验单位≥2×105个细胞时,ATP浓度随细胞数的增加而增加,有一线性关系;不同浓度(1x、2x、5x×plasmapeakconcentration)的顺铂、阿霉素作用于L929细胞系,前者有明显的剂量-反应关系,后者无。由此表明ATP分光光度法对于了解恶性肿瘤的能量代谢,以及化疗药物对其影响是一种价廉的可行的方法。 To evaluate the value of ATP spectrophotometry for malignant tumor in vitro chemosensitivity test, Hela and L 929 cell lines, which were derived from human cervical carcinoma and mouse fibroma respectively, were investigated. The results showed that when the cellular numbers were≥2×10 5, the linearity between the ATP concentrations and the cellular numbers was observed, with all 2 cell types studied, and that when L 929 cells were incubated with cis platinum(1x, 2x, 5x×plasma peak concentration, PPC), a marked fall in ATP concentrations was observed and a clear dose response curve was obtained, but no decrease of ATP concentrations was measured after incubation with adriamycin(1x, 2x, 5x×PPC). This study suggests that ATP spectrophotometry is a cheap, feasible method for studying the energy metabolism and the influence of chemotherapeutic drugs on ATP concentrations of malignant tumor.
出处 《华西医科大学学报》 CSCD 1997年第3期300-303,共4页 Journal of West China University of Medical Sciences
关键词 ATP含量 分光光度法 药敏试验 恶性肿瘤 Spectrophotometry Adenosine triphosphate Malignant tumor Chemosensitivity test
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