化学发光法测定大黄素大黄酚的淬灭系统研究

Optimization of Quenching System for Determination of Emodin and Chrysophanol by Chemiluminescence Method

复方中成药制剂中有效成分的含量测定是鉴定中成药质量的关键之一，尤其是准确简便的方法更是当前药品检验工作所需要的。作者经优选试验，以鲁米诺－Ｈ２Ｏ２－Ｃｒ３＋为化学发光淬灭系统，确定了测定大黄素和大黄酚的最佳测定条件和操作步骤。测得大黄素的线性范围为１０－４～１０－８ｇ／ｍｌ（ｒ＝０．９９６５），检出限为１．５７×１０－９ｇ／ｍｌ；测定１０－７ｇ／ｍｌ大黄素时，ＲＳＤ为０．４％，回收率为９２．８±０．５％（ｎ＝６）。测定大黄酚可以得到同样满意的结果。本法用于经薄层分离后的中成药中大黄素、大黄酚的测定，结果与标准比色法一致。

Determination of the valid components in traditional Chinese medicine is one of the key steps for its identification,and the development of accurate and simple methods is necessary for the present analysis work of medicines.In this paper,the experimental conditions were optimized and procedures chosen for determination of emodin and chrysophanol by lunminol H 2O 2 Cr 3+ chemiluminescence quenching system.The dynamic range for determination of emodin was 10 -4 10 -8 g/ml ( r=0.9965) , with a detection limit of 1.57 ×10 -9 g/ml.The RSD and recovery for determination of 10 -7 g/ml of emodin ( n=6) were 0.4% and 92.8%±0.5%,respectively.Satisfactory results were also obtained when the method was used to determine chrysophanol.This method was used,after separation by thin layer chromatography,to determine emodin and chrysophanol in several traditional Chinese patent medicines,and the results were in good agreement with those obtained by the standard colorimetric method.