尖锐湿疣组织LTBP-1和TGF-βRⅡ的表达

Expression of latent transforming growth factor beta binding protein-1 and transforming growth factor beta type Ⅱ receptor in condyloma acuminatum tissues

目的探讨潜在结合蛋白（LTBP）-1及其Ⅱ型受体（TGF—βRⅡ）与尖锐湿疣发病的相关性。方法采用实时荧光定量PCR技术和SP免疫组化技术分别检测30例CA皮损及17例正常包皮组织中LTBP和TGF-βRⅡ的mRNA和蛋白的表达。结果CA皮损中LTBP-1及TGF-βRⅡ的mRNA的表达水平均低于对照组包皮组织（2^-△△α〉2）。免疫组化结果显示：LTBP-1和TGF—βRⅡ的染色强度较正常人包皮组织降低，差异有统计学意义（P〈0．01）：结论LTBP-1和TGF—βRⅡ的表达下调，可导致TGF—β活化及信号转导通路的异常.

Objective To investigate the role of the expression of latent transforming growth factor beta binding protein-1 （LTBP-1） and transforming growth factor beta receptor type Ⅱ （TGF-beta R Ⅱ ） in the pathogenesis of condyloma acuminatum （CA）. Methods Samples were resected from the lesions of 30 patients with CA and prepuces of 17 normal human controls. The mRNA and protein expressions of LTBP-1 and TGF-betaR Ⅱ were assessed by quantitative real-time PCR and a streptavidin-biotin-peroxidase staining technique, respectively. Results As shown by Real time PCR, the mRNA expression levels of LTBP-1 and TGF-betaR Ⅱ were significantly higher in CA tissues than those in the controls, with the average value of 2 ^（-Delta Delta α） being 2.46 and 3.43, respectively. A lower intensity of stainning was observed for LTBP-1 and TGF-betaR Ⅱ in CA tissues compared with the normal controls （182.51 ± 9.89 vs 167.78 ± 12.56, 187.35 ± 11.23 vs 170.15 ± 13.21, t = 5.62, 3.70 respectively, both P 〈 0.01）. Conclusion The decrease in the expression of both LTBP-1 and TGF-betaRⅡ may lead to the abnormality in the activation of TGF-beta and signal transduction pathways.