正交设计法优选千斤拔总生物碱的提取工艺

Study on extration process of whole alkaloid of Moghania philippinensis(Merr. et Rolfe)Li by orthogonal design method

目的:筛选千斤拔药材中总生物碱的提取工艺.方法:以总生物碱的最大吸光度值为指标,采用正交实验设计法筛选生物碱的提取方法及其工艺参数.结果:千斤拔中总生物碱的最佳提取工艺为:酸水渗漉提取法,最佳提取工艺参数为硫酸浓度为3%,渗漉时间为12 h,流速为1 ml.min-1.结论:采用该提取方法,可以得到较高产率的生物碱类成分;符合工业化生产要求.

Objective： To screen the extraction and purification technology of whole alkaloid from Moghania philippinensis （Merr. et Rolfe）Li. Methods： Designed L9（34） orthogonal test with rutin as index to determine the reasonable extract technology. Result： The best extract technology is acid water diacolation method with 1 ml·min^-1 percolate speed, using 16 times 3 % acid water as solvent, and immersing 12 hours as the best extract plan. Conclusion： The extract technology is suitable for industrialized production.