雌孕激素对STAT3在小鼠子宫内膜表达的调控

Hormonal regulation of STAT3 expression and activation in mouse uterine endometrium

目的:探讨雌、孕激素对STAT3在小鼠子宫内膜表达的影响。方法:将小鼠卵巢切除,随机分为对照组(腹腔注射油)、E2组(腹腔注射苯甲酸雌二醇)、P组(腹腔注射黄体酮)和E2+P组(腹腔注射苯甲酸雌二醇和黄体酮)。用RT-PCR方法检测STAT3 mRNA在各组小鼠子宫内膜的表达。同时用免疫组化方法检测STAT3和磷酸化STAT3蛋白在各组小鼠子宫内膜的表达。结果:STAT3 mRNA在E2组和E2+P组有明显的表达,而在P组和对照组无表达。STAT3蛋白在E2组和E2+P组有明显的表达,而在P组和对照组无表达。P-STAT3蛋白在E2组有强表达;在P组和E2+P组也有明显的阳性表达;而在对照组则无表达。结论:在小鼠子宫内膜,雌激素促进STAT3 mRNA和蛋白的表达;孕激素无促进STAT3 mRNA和蛋白表达的作用,但可以促进STAT3的磷酸化。在胚胎种植的过程中,STAT3的表达和活化受到雌、孕激素的调控。

Objective： To investigate hormonal regulation of STAT3 expression and activation in mouse uterine endometrium. Methods： The ovarieetomized mice were divided into four groups. The control group was treated with oil, the E2 group was treated with estradiol benzoate, the P group was treated with progesterone, the E2 ＋ P group was treated with estrogen and progesterone. The RT - PCR was used to detect the expression of STAT3 mRNA in the uterus of the four groups. Immunohistoehemistry was used to detect the expression of STAT3 and P - STAT3 in the uterine endometrium of the four groups. Results： STAT3 mRNA expression was strongly detected in the uterus of the E2 group and the E2 ＋ P group, while there was no STAT3 mRNA expression in the uterus of control group and P group. High level of STAT3 was detected in uterine endometrium of E2 group and E2 ＋ P group, while STAT3 was not detected in uterine endometrium of control group and P group. P - STAT3 was not detected in uterine endometrium of control group, while it was detected in uterine endometrium of other three groups in high level. Conclusion： The expression of STAT3 mRNA and protein in the uterine endometrium were stimulated by estregen. Progesterone has no use in this, but it can stimulate the phosphorylation of STAT3. Estrogen can also stimulate the phosphorylatian of STAT3.