日本血吸虫循环免疫复合物中抗原的鉴定

IDENTIFICATION OF ANTIGENS IN CIRCULATING IMMUNE COMPLEXES OF SCHISTOSOMIASIS JAPONICA

用低浓度ＰＥＧ（ＭＷ６０００）提取的日本血吸虫感染兔血清中循环免疫复合物（ＣＩＣ）直接免疫家兔，制备抗ＣＩＣ兔血清。采用酶联免疫印迹技术（ＥＩＴＢ）分析鉴定日本血吸虫ＣＩＣ中抗原成份。结果表明，ＣＩＣ中成虫及其排泄分泌物源性抗原成份有１７种，虫卵源性抗原成份有５种。用ＩＦＡＴ方法分析ＣＩＣ中抗原成份定位于成虫表膜及肠腔上皮。

In order to identify antigens in circulating immune complexes(CIC) of Schistosoma japonicum, the antiserum against CIC was first successfully prepared by immunization of New Zealand rabbit with CIC isolated from schistosome-infected rabbit sera. The antigens in CIC were identified and analyzed by EITB(enzyme linked immunoelectro transfer blot) techniques, using the prepared antiserum against CIC, adult worm antigens (AWA), soluble egg antigens(SEA) and adult excretory antigens(ESA). The results showed that the antiserum against CIC had recognized the AWA bands of 155, 90, 70, 31/32, 20.5, 14 and 8KD; the SEA bands of 90, 66, 46, 34.5 and 27KD and the ESA bands of 155, 92, 87.5, 70, 60, 57.5, 50, 46, 36, 31/32, 24, 18.5, 12 and 8KD. These findings indicate that the antigens in CIC were derived from both schistosome adult worms and eggs. In addition, the localization of the antiserum against CIC targeted antigens in adult worms was also carried out by IFAT(indirect flourescent antibody test). It is suggested that the antigens in CIC were presented mainly in the tegument and the gut of the parasite.