摘要
采用原位多聚酶链反应技术检测了５例喉癌石蜡组织切片中ｐ５３基因，结果在全部标本中９０％以上的癌细胞核检测到ｐ５３基因，平均拷贝数为２．２４±０．２３。表明该技术可用于单拷贝基因研究，并对其改进和应用进行讨论。
In order to investigate alterations of p53 gene in laryngeal carcinoma, five cases of paraffin embedded tissue were detected by in situ polymerase chain reaction(PCR). The results showed that p53 gene was presented in more than 90% of cancer cells in all cases with exon 5 specific primers, which signal copy numbers were 2.24±0.23. It suggests that in situ PCR and paraffin embedded tissue are able to be used in the single copy gene research. The improvement and application of the technique were discussed.
出处
《湖南医科大学学报》
CSCD
1997年第5期459-461,共3页
Bulletin of Hunan Medical University
基金
湖南省卫生厅1994年重点项目
国家教委留学回国人员启动基金
关键词
喉肿瘤
多聚酶链反应
P53基因
laryngeal neoplasms
carcinoma
gene
p53
polymerase chain reaction
methods
