低锌与缺锌胁迫时玉米基因表达差异的研究

Research on Differentially Expressed Genes in Maize Under Low-zinc and Zinc-deficiency Conditions

利用抑制差减杂交(Suppression Subtractive Hybridization,SSH)技术分离玉米低锌与缺锌胁迫时表达有差异的cDNA片段。以低锌培养的玉米幼苗为实验方,缺锌培养的玉米幼苗为对照方,建立正向差减文库;以缺锌培养的玉米幼苗为实验方,以低锌培养的玉米幼苗为对照方,建立反向差减文库。正、反向文库分别收集低锌和缺锌条件下特异表达的cDNA片段。在两个文库中,各随机挑选15个克隆进行测序。序列分析表明,玉米在受到低锌和缺锌胁迫时所表达的基因有所不同。这些差异表达基因为解释低锌和缺锌胁迫时玉米受害的不同表现提供了进一步的依据。

Suppression subtractive hybridization（SSH） was performed to isolate the differentially expressed cDNA fragments on maize under low-zinc and zinc-deficiency conditions. In the forward-subtracted library, the cDNA of maize seedlings treated with low-zinc was used as tester and the cDNA of maize seedlings treated with zinc-deficiency was used as driver. In the reverse-subtracted library, the cDNA of maize seedlings treated with zinc-deficiency was used as tester and the cDNA of maize seedlings treated with low-zinc was used as driver. The cDNA fragments differentially expressed under low-zinc and zinc-deficiency conditions were collected in the forward and reverse subtracted library, respectively. Fifteen clones were randomly picked to sequence in each library. The sequencing results showed that the genes induced under low-zinc and zinc-deficiency conditions were different. The differentially expressed genes could be used to explain the different appearances when maize seedlings were treated with low-zinc and zinc-deficiency.