玉米丝黑穗病菌的PCR检测

PCR Detection of Sporisorium Reilianum

根据玉米丝黑穗病菌pra3基因序列设计了1对扩增玉米丝黑穗病菌的特异引物。结果显示,用该特异引物扩增玉米丝黑穗病菌DNA可以得到一条大小为499bp的目的条带,扩增玉米丝黑穗病菌DNA的灵敏度可以达到1pg,建立的PCR反应可用于玉米丝黑穗病菌的检测。

A pair of species-specific primer for sporisorium reilianum was designed according to the pra3 gene of sporisorium reilianum, and the results of polymerase chain reaction showed that a 499bp DNA was amplified by using the primers, and the PCR-hased assay could detect more than lpg fungal DNA using the primer. The PCR as a highlysensitive and reliable tooi, Could be used in detection of sporisorium reilianum.