润肠口服液的薄层色谱鉴别

Identifying Moistening Intestines Orally Liquid by Thin-Layer Chromatography

目的:采用薄层色谱法(TLC)鉴别润肠口服液中黄芪、白芍、当归。方法:黄芪鉴别以氯仿-醋酸乙酯-甲醇-水(20∶40∶22∶10)10℃以下放置后的下层液为展开剂,以硫酸乙醇溶液(1→10)显色剂;白芍鉴别以氯仿-醋酸乙酯-甲醇-甲酸(40∶5∶10∶0.2)为展开剂,以5%香草醛硫酸溶液为显色剂;当归鉴别以正己烷-醋酸乙酯(9∶1)为展开剂。结果:各色谱斑点清晰,分离度好,阴性对照无干扰。结论:该鉴别方法简便、可靠、灵敏、专属性强,可用于润肠口服液的质量控制。

Objective： to identify the astragalus mongholicns, white peony root and angelica root in the moistening intestines orally liquid by TLC. Method： the developing agent was the subnatant in identifying astragalus mongholicus by chloroform-ethyl acetatemethanol-water （20：40：22：20） under 10℃ and developer was sulphuric acid-alcohol（1 ： 10）. The developing agent was chloroformethyl acetate-methanol-formic acid（40：5 ： 10 ： 0.2）and developer was citroella acid solution in identifying white peony root. The developing agent was N hexane-ethyl acetate（9：1）. Result： the color spectrum spot was clear and well resolving power. The negative control had non-interference. Conclusion： this method may use to quality control to moistening intestines orally liquid for convenient, reliability, sensitive and specificity.