Ⅱ型犬腺病毒C株E1、E3区克隆测序及E3区标记载体瞬时表达

Sequence of E1 and E3 of Canine Adenovirus Type II C Strain and Transient Expression of Transfer Deleted Vector E3 with EGFP

设计4对引物,对犬Ⅱ型腺病毒2C(CAV-2C)株E1、E3区进行了扩增,将片段进行T克隆、测序,并构建了E3区缺失、标记EGFP的转移载体,在MDCK和293-T细胞上成功表达,但在MDCK细胞中的转染效率较低。本研究为犬腺病毒活病毒载体构建的研究提供基础。

Four pairs of primers were designed to amplify E1 and E3 of CAV-2C. We cloned these fragments into pMD18-T Vector, which was subsequently sequenced. We constructed transfer vector deleted E3 with EGFP marker gene. Then vector expression was carried out in MDCK and 293-T. The results showed that the transfection efficiency in MDCK was very low, while higher in 293-T cell. We laid foundation in this study for adenovirus vector construction.