细菌细胞表面疏水性及在活性污泥中粘附率影响因素研究

Factors Influencing Bacterial Cell-surface Hydrophobicity and Bacterial Adhesion Ratio to Activated Sludge Flocs

以4株不同菌株为对象,研究了培养基类型对细菌细胞表面疏水性的影响,及初始细胞表面疏水性、接触时间等因素与细菌在活性污泥中粘附率的关系.结果表明,培养基类型、培养时间以及菌株的自身特性均会对所培养菌种的表面疏水性产生影响;而初始细胞表面疏水性、接触时间与细菌在活性污泥中的粘附率密切相关.当接触时间较短时(<14h),初始细胞表面疏水性是影响系统中菌种粘附量的主要因素,且存在某一临界值,当菌种的疏水率低于该值时,菌种粘附量相近,当菌种的疏水率高于该值时,粘附量明显增高,菌种可以迅速粘附到活性污泥中;而当接触时间足够长时(≥38h),接触时间则成为系统中菌种粘附率的主要影响因素.经预接触,外投高效菌被系统完全吸附后,营养物质的投加或供氧方式的改变均不会引起被吸附菌种的再释放.

With four different strains as the model, effects of different culture medium on cell-surface hydrophobicity （CSH） were investigated, and the relationship between CSH, contact time and adhesion of bacteria to activated sludge flocs was correlated. Results showed that culture medium, culture time and the characteristic of strain itself influenced the CSH of bacteria, and initial CSH, contact time was closely related to bacterial adhesion ratio in activated sludge system. When contact time was relatively short （〈14 h）, initial CSH was the main factor infuencing bacterial adhesion ratio. A critical value existed for CSH, similar bacterial adhesion ratio was obtained below the critical value; beyond the value, bacterial adhesion ratio was greatly enhanced, which indicated that only strains with initial CSH beyond the critical value could adhere to activated sludge flocs rapidly. When contact time was long enough（ ≥38 h）, contact time became the main factor influencing adhesion. When the inoculated bacteria thoroughly adhered to sludge flocs by pre-contact, nutrient supplementation and changing oxygen providing mode did not cause the release of adhered bacteria again.