固定化氨氧化细菌短程硝化稳定性研究

Stability of Short-cut Nitrification Using Immobilized Ammonia-oxidizing Bacteria

以高分子聚合物为载体,采用细胞增殖技术固定氨氧化细菌,研究了氨氮负荷、HRT、初始游离氨(FA)和有机物等因素对短程硝化过程稳定性的影响.实验结果表明,当进水氨氮负荷分别为100、150和200mg/L时,出水氨氮浓度均小于10mg/L;当进水氨氮浓度为25.8、51.1和93.3mg/L时,分别经历3、6、12h后,出水氨氮浓度低,亚硝化效果好,可以根据进水氨氮浓度的变化,适当地调整系统水力停留时间(HRT)并优化系统的运行;当游离氨(FA)浓度>9mg/L时,对氨氧化细菌会产生抑制;低分子有机物的存在对氨氧化细菌的活性具有一定的促进作用,有机物浓度对亚硝化率基本不产生影响,实验过程中在有机物存在的条件下,发生了短程硝化反硝化反应,使得系统总氮减少.

The ammonia-oxidizing bacteria were immobilized by copolymer with cell proliferation technology. The effects of NH4^＋ -N load, HRT, free ammonia（FA） and organic matter on short-cut nitrification process were studied. The results showed that when influent NH4^＋ -N load were 100 mg/L, 150 mg/L and 200 mg/L respectively, effluent NH4^＋ -N concentration was less than 10 mg/L. When the system run for 3 h, 6 h and 12 h, corresponding to influent NH4^＋ -N concentration of 25.8 mg/L, 51.1 mg/L and 93.3 mg/L respectively, NH4^＋ -N concentration was low in effluent with high short-cut nitrification efficiency. The HRT could be adjusted to optimize the system operation with variation of influent NH4^＋ -N concentration. The results also indicated that the ammonia-oxidizing bacteria were restrained while free ammonia concentration was over 9 mg/L. The activity of the ammonia-oxidizing bacteria could be enhanced under existence of low-molecular-weight organic compounds, but the short-cut nitrification efficiency was little affected. In addition, the short-cut nitrification and denitrification could be realized with existence of organic compounds during the experiment.