壳聚糖改性、抑菌性及成膜性研究

Preparation of Carboxymethyl Chitoson Modification and Its Film-forming and Bacteriostasis

对壳聚糖通过改性,制备易溶于水的壳聚糖衍生物——羧甲基壳聚糖,并对它的成膜性、抑菌性进行研究。本实验主要以氯乙酸为改性剂,以异丙醇为反应溶剂制备羧甲基壳聚糖,探讨了加碱量、反应时间、投料比和反应温度等工艺条件对壳聚糖羧甲基化程度的影响及不同投料比制备的羧甲基壳聚糖和不同浓度的羧甲基壳聚糖溶液的成膜性、抑菌性。羧甲基化壳聚糖最佳反应条件为:碱浓度50%、反应温度50℃,反应时间3 h,壳聚糖与氯乙酸的质量比为1∶4.5,粘度46mPa.s,取代度0.74DS,溶液澄清,数分钟后溶解。浓度为1%左右时,成膜效果最佳。羧甲基壳聚糖对大肠杆菌有抑制作用。浓度为0.2%时已有抑菌性。

This paper disscusses chitoson ramification of water-soluble bacteriostasis and water-soluble ability. In this experiment chloroactic medium. And it has influence on different technological conditions, for carboxymethyl chitoson, and researches its is modifier and isopropanol as reaction example, time and temperature of reaction and adding different reagent and so on. The optimum condition of this experiment is： sodium hydroxide concentration 50%, the time of reaction is 3 hours, the temperature of reaction is 50%, m（CYS）： m（chloroactic acid） = 1：4.5 ,Viseosity 46mpa · s,DS 0. 74, aqueous solution viscosity of carboxymethyl chitoson in 1% has film forming. N, O-carboxymethyl chitosan has anti-Escherichia coli action in 0.2%.