摘要
目的:对牛蒡药材不同部位牛蒡子苷和牛蒡子苷元含量测定。方法:采用高效液相色谱法的测定。Agilent 1100液相色谱系统四元泵,Agilent色谱柱(4.6mm×250mm,5μm);甲醇-水二元梯度洗脱,检测波长280nm。结果:牛蒡的根、茎、叶中几乎不含牛蒡子苷和牛蒡子苷元,绒毛和花序中含少量牛蒡子苷和牛蒡子苷元,而牛蒡子在牛蒡中二者含量均高。结论:该方法简便、准确,为更好的开发利用牛蒡提供依据。
Objective :To establish a ItPLC method for determination of of different spots of Arctium Lappa L. Methods :The HPLC method was employed by taking Agilent column as fixed phase and methanol - water as mobile phase. Results :It's root,stem and leaf nearly do not contain,the down and the inflorescence contain few aretiin and arctigenin ,but the two contents are highest in the fruit. Conclusion :The method is simple, accurate. It provides the basis for a better development of Arctium Lappa L.
出处
《中华中医药学刊》
CAS
2008年第10期2160-2161,共2页
Chinese Archives of Traditional Chinese Medicine
基金
国家"十一五"科技支撑计划项目(2006BAI09B02-6)