8种中药醇提取液对两种自由基抑制能力的测定方法

Study on Ability of Extraction Alcohol from Eight Kinds Chinese Traditional Medicine in Inhibiting Two Free Radicals

目的:探讨中药的醇提取液对两种自由基的抑制作用,为研制抗衰老药物奠定基础。方法:用邻苯三酚法测定8种中药醇提取液对超氧阴离子自由基(O2-)的抑制作用,用Fon ten反应检测8种中药醇提取液对羟自由基(HO)的抑制作用。结果:8种中药醇提取液对O2-的抑制能力为:五味子>绿茶>甘草>黄芪>川芎>熟地>党参>丹参;对HO的抑制能力为:绿茶>五味子>甘草>川芎>熟地>党参>丹参>黄芪。结论:8种中药醇提取液对O2-和HO均有清除作用。

Objective： To study on the ability of extraction alcohol from eight kinds Chinese traditional medicine in inhibiting two free radicals,and to provide the solid foundation for the development of anti-old medicine. Methods ： Pyrogollol self-oxidation was employed to measure the ability of extraction alcohol from eight kinds Chinese traditional medicine in inhibiting two free radicals to super-oxide radical. Fonten Reaction was employed to test the ability of inhibition of extraction alcohol to hydroxyl radical. Results.. （1）The ability of extraction alcohol from eight kinds Chinese traditional medicine in inhibiting two free radicals to super-oxide radical could be ranked as.-fructus schizandrae〉green tea〉 radix glycy：rrhizae 〉radix astragali〉rhizome ligusitc〉radix et rhizome rehmanniae 〉radix codonopsitis〉radix salviae miltiorrhize. （2）The ability of extraction alcohol from eight kinds Chinese traditional medicine in inhibiting two free radicals to hydroxyl radical could be ranked as：green tea〉fructus schizandrae〉radix glycyrrhizae〉rhizome ligusite〉radix et rhizome rehmanniae〉radix codonopsitis 〉radix salviae miltiorrhize〉fructus schizandrae. Conclusions ：Extraction alcohol from eight kinds Chinese traditional medicine possess the ability of clearance to both super-oxide radical and hydroxyl radical.