结直肠癌患者骨髓微小转移的免疫组化研究

Micrometastasis of colorectal carcinoma in bone marrow with immunohistochemical staining

为研究直肠癌的骨髓微小转移，自１９９４年４月至１９９６年４月作者采用抗ＥＭＡ单抗检测直、结肠癌患者骨髓血中的微小转移灶。全组共５７例，３４例结肠癌患者检出１４例阳性病例（阳性率为４１％），２３例直肠癌发现９例阳性病例（阳性率为３９．１％），经统计学分析发现阳性细胞检出率与患者年龄、癌肿病理类型及改良Ｄｕｋｅｓ分期有关，而与癌的原发灶部位无关。作者认为该法可准确判断直、结肠癌的病变范围，指导临床治疗有重要意义。

From April 1994 to April 1996,immunohistochemical technique was used to detect micrometastasis of patients with colorectal carcinoma with monoclonal antibody to epithelial membrane antigen (anti EMA).Positive cells were found in their bone marrow in 23 of 57 cases,including 14 in 34 patients with colonic carcinoma(positive rate 41%) and 9 in 23 patients with rectal carcinoma(positive rate 39 1%).The positive rate was 40 4%. Statistical analysis showed that the modified Dukes stage of the cancer,the pathological type of carcinoma and the age of the patients were correlated with the positive rate, but the primary locations of the cancer were not.This method can be used to identify the extent of the cancer and direct clinical comprehensive treatment for colorectal carcinoma.