睾丸畸胎瘤核仁组成区嗜银蛋白定量研究

Nucleolar organizer regions enumeration in testicular teratoma

为进一步探讨睾丸良恶性畸胎瘤的鉴别方法，作者应用核仁组成区银染技术，对１０例睾丸正常组织、６例睾丸畸胎瘤、７例睾丸畸胎癌进行了研究。结果显示：正常睾丸及畸胎瘤的ＡｇＮＯＲｓ颗粒呈圆形、规则、边界清楚、大小均匀，位于胞核偏中或边缘部位；畸胎癌的颗粒呈圆形或卵圆形、欠规则、边界欠清、大小较不一致，偏中或散在分布于核内。ＡｇＮＯＲｓ计数：正常组为１．５６±０．１７，畸胎瘤为２．４０±０．２６，畸胎癌为５．２４±０．３６，各组间有显著差异（Ｐ＜０．０１），ＡｇＮＯＲｓ计数在良恶性畸胎瘤间有分离。作者认为该技术对睾丸良恶性畸胎瘤的鉴别是有意义的。

To differenciate the benign and malignant testis teratoma.We investigated the value of AgNORs enumeration in 10 normal testis tissues, 6 benign teratomas and 7 malignant teratomas.The shape of the AgNORs in normal and benign teratoma was round, regular,with clear boundary and even size, setting in the center or the margin of the nucleus.In the malignant teratoma, the shape was irregular with unclear boundary and uneven size, setting in the near center or scattered in the nucleus.AgNORs enumeration:normal group:1 56 ± 0 17, benign teratoma: 2 40±0 26, malignant teratoma: 5 24±0 36. There was significant difference between the two groups ( P <0 01).The range of AgNORs enumeration in benign and malignant teratoma was separate.These results indicate that the mathod is helpful in differentiating benign and malignant testicular teratoma,and may provide an objective parameter.