凋亡相关基因bcl-2和bax蛋白在膀胱癌组织中的表达及临床意义

Expression of the bcl 2 and bax oncoprotein in TCC and its clinical significances

为探讨凋亡抑制基因ｂｃｌ－２和凋亡促进基因ｂａｘ表达产物在膀胱癌组织中的分布及意义，作者应用抗ｂｃｌ－２单克隆抗体和抗ｂａｘ多克隆抗体分别对３４例膀胱癌和９例正常膀胱粘膜组织的石蜡切片进行免疫组化染色。结果显示，９例正常膀胱粘膜有４例ｂｃｌ－２蛋白表达阳性（４４．４４％），３４例膀胱癌有２８例ｂｃｌ－２蛋白表达阳性（８２．３５％），两组间差异有显著意义（Ｐ＜０．０５），随肿瘤分级的增加，阳性表达增强。９例正常膀胱粘膜有８例ｂａｘ蛋白表达阳性（８８．８９％），３４例膀胱癌有１８例表达阳性（５２．９４％），两组间差异有显著意义（Ｐ＜０．０５）。作者认为，ｂｃｌ－２和ｂａｘ蛋白在膀胱癌组织中的异常表达，参与了膀胱癌的发生及进展过程。

To investigate the role of bcl 2(an apoptosis suppressing oncogene) and bax(an apoptosis accelerating oncogene) in the development of TCC.We investigated bcl 2 and bax expression by means of immunohistochemical technique in 34 cases of TCC and in 9 cases of normal bladder tissue. Bcl 2 was positive in 44 44% of normal bladder tissue and in 82 35% of TCC. Bcl 2 staining intensity was significantly stronger in TCC than that in normal bladder tissue. Intensity and positivity of bcl 2 also increased with increasing grades of TCC. Bax was positive in 88 89% of normal bladder tissue and in 52 94% of TCC. Bax staining intensity was significantly weaker in TCC than that in the normal bladder tissue. These results suggested that increased expression of bcl 2 and decreased expression of bax in TCC play an important role in the development of TCC.