Beckman Coulter HMX血细胞分析仪性能评价

Evaluation on performance of Beckman-Coulter HMX hematocyte analyzer

目的在了解美国贝克曼-库尔特公司生产的HMX型(Beckman Coulter HMX)血细胞分析仪的精密度、准确度、携带污染率的同时,对该仪器白细胞分类及网织细胞计数与手工法比较的相关性及可疑报警提示观察,以评价其性能。方法选用定值指控全血连续重复测定,选取白细胞、红细胞、血小板计数及血红蛋白测定4个主要参数以评价仪器精密度及准确度;高、低值样品连续重复3次评价仪器的携带污染率。选取门诊及住院患者全血标本30例观察白细胞分类,选30例标本做网织红细胞计数的检测,仪器法与手工法比较,另外选取住院患者标本30例,对异常细胞报警提示进行分析。结果质控物测定结果CV<2.24%;携带污染率小于1.13%;仪器法与手工法比较白细胞分类P>0.05;网织红细胞计数P>0.05;报警提示P<0.05。结论美国贝克曼-库尔特公司生产的HMX型血细胞分析仪准确性、精密度均良好,携带污染率低;白细胞分类及网织红细胞计数仪器法与手工法比较相关性较好;异常细胞报警提示功能敏感度高,可作筛查检测使用。

Objective To perform a technical evaluation on performance of Beckman-Coulter HMX hematocyte analyzer in regard to its accuracy, precision and carry over rate; the correlation in leukocyte differential count and reticulocyte count between the analyzer method and manual method as well as doubtful warning. Methods Rating quality control whole blood was selected to measure four parameters （WBC, RBC,PLT and HB） successively and repetitively to evaluate the precision and aecuracy; high- and low-value serum specimens were determined for successive 3 times to investigate the carry over rate. 30 whole blood samples were collected from outpatients and inpatients for WBC diff and reticulocyte count. Besides, additional 30 whole blood samples were collected from inpatients to study the abnormal cell warning. Results The detection result of quality control specimen showed CV was below 2.24%, and the carry over rate was below 1.13%. There weren＇t statistical differences in leukocyte differential count and reticulocyte count between analyzer method and manual method （P〉0. 05）. There was statistical significance in warning hint （P〈0.05）. Conclusion Beckman-Coulter HMX hematocyte analyzer is of high precision and accuracy, low carryover rate, and sensitive abnormal cell warning hint function, closely correlative to manual method. Its favorable performance makes it an available analyzer for hematocyte monitoring and detecting.