聚合酶链反应在MLCK表达载体构建中的应用

Application of polymerase chain reaction in construction of MLCK expression vector

用聚合酶链反应（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ，ＰＣＲ）方法扩增出含有ＳａｌⅠ位点和转录起始点ＡＴＧ的肌球蛋白轻链激酶（ｍｙｏｓｉｎＬｉｇｈｔｃｈａｉｎｋｉ－ｎａｓｅ，ＭＬＣＫ）ｃＤＮＡ片段，并插入质粒ｐＢＫｒｓｖ中，构建成能在宿主细胞中表达的表达载体，并通过酶切图谱、ＳａｌⅠ位点ＤＮＡ序列分析得到证实。为进一步研究ＭＬＣＫ的表达奠定基础。

A cDNA fragment of myosin light chain kinase (MLCK) containing SalⅠsite and ATG was generated by polymerase chain reaction (PCR). The pBKrsv MLCK was constructed by inserting MLCK cDNA into pBKrsv polylinker and identified by restriction mapping sequence analysis of SalⅠ site. This work provides the foundation for further investigation of MLCK exprssion MeSH polymerase chain reaction (PCR); myosin light chain kinase (MLCK); pBKrsv; restriction endonuclease mapping assay; DNA