摘要
研究高密度脂蛋白-3(HDL3)氧化修饰对其介导大鼠腹腔巨噬细胞胆固醇(ch)流出功能影响及细胞Ch流出机制。HDL3和其组分载脂蛋白A-I(ApoA-I)经CU2+氧化修饰后分别与巨噬细胞(Mφ)一起培养,测定细胞ch流出量。结果:牛血清白蛋白(对照组)介导3.98%细胞ch流出,HDL3和氧化HDL3(Ox—HDL3)组分别为41.78%和17.43%,ApoA—I和Ox-ApoA一I分别为40%和17.33%。结论:1.HDL氧化修饰后介导细胞ch流出功能显著降低。2.HDL的这一功能是由其ApoA—I组分介导的。
PURPOSE To study the effect of oxidative modification of high density lipoprotein (HDL) on cholesterolefflux from rat macrophages and its mechanism.METHODS To oxidize HDL3 and ApoA-Ⅰ by Cu2+, incubate with rat macrophages, and determinecholesterol efflux from the cells.RESULTS BSA(control group) mediated 3. 98 % of cell's cholesterol efflux from the cells. HDL3 and Ox-HDL3 group mediated 41. 78 % and 17. 43 % respectively; ApoA-Ⅰ and Ox-ApoA-Ⅰ group were 40 %and 17. 33 % respectively.CONCLUSIONS 1. Oxidative modification of HDL3 weakens significantly its capacity of mediating cholesterol efflux from macrophages (P<0. 05). 2. The function of HDL is mediated by its ApoA-Ⅰ component.
出处
《上海医科大学学报》
CSCD
1997年第5期335-338,共4页
Journal of Fudan University(Medical Science)
基金
国家自然科学基金!39570158
关键词
巨噬细胞
胆固醇流出
高密度
脂蛋白
氧化修饰
rat macrophages
cell cholesterol efflux
oxidative modification of high density lipoprotein