高密度脂蛋白氧化修饰对大鼠巨噬细胞胆固醇流出的影响及其机制

EFFECT OF OXIDATIVE MODIFICATION OF HIGH DENSITY LIPOPROTEIN ON CHOLESTEROL EFFLUX FROM RAT FACROPHAGE AND ITS MECHANISM

研究高密度脂蛋白-3（HDL3）氧化修饰对其介导大鼠腹腔巨噬细胞胆固醇（ch）流出功能影响及细胞Ch流出机制。HDL3和其组分载脂蛋白A－I（ApoA－I）经CU2+氧化修饰后分别与巨噬细胞（Mφ）一起培养，测定细胞ch流出量。结果：牛血清白蛋白（对照组）介导3.98％细胞ch流出，HDL3和氧化HDL3（Ox—HDL3）组分别为41．78％和17．43％，ApoA—I和Ox－ApoA一I分别为40％和17．33％。结论：1．HDL氧化修饰后介导细胞ch流出功能显著降低。2．HDL的这一功能是由其ApoA—I组分介导的。

PURPOSE To study the effect of oxidative modification of high density lipoprotein (HDL) on cholesterolefflux from rat macrophages and its mechanism.METHODS To oxidize HDL3 and ApoA-Ⅰ by Cu2+, incubate with rat macrophages, and determinecholesterol efflux from the cells.RESULTS BSA(control group) mediated 3. 98 % of cell's cholesterol efflux from the cells. HDL3 and Ox-HDL3 group mediated 41. 78 % and 17. 43 % respectively; ApoA-Ⅰ and Ox-ApoA-Ⅰ group were 40 %and 17. 33 % respectively.CONCLUSIONS 1. Oxidative modification of HDL3 weakens significantly its capacity of mediating cholesterol efflux from macrophages (P<0. 05). 2. The function of HDL is mediated by its ApoA-Ⅰ component.