电惊厥大鼠海马生长抑素神经元的超微结构变化

ULTRASTRUCTURA CHANGES OF HIPPOCAMPAL SOMATOSTATIN MEURONS IN THE RAT FOLLOWING ELECTROCONVLSIVE SHOCK

观察电惊厥大鼠海马生长抑素神经元的超微结构变化，为探讨生长抑素在癫痫发病中的作用机制提供形态学依据。采用电惊厥癫痫动物模型和免疫电镜方法观察。结果：电惊厥时海马内生长抑素神经元胞体和突起出现不同程度的结构损伤，表现为线粒体肿胀、细胞器崩解和神经末梢变性等；在海马区多形细胞层和分子层生长抑素神经元胞体、突起与非生长抑素神经元胞体、突起之间有复杂的突触联系。结论：电惊厥时海马CA4生长抑素神经元结构发生损伤。

PURPOSE To observe the ultrastructural changes Of hippocampal somatostatin(SS) neurons in the rat following electroconvulsive shock (ECS), and to investigate the role of hippocampal SS in experimental epilepsy.METHODS The epileptic model was induced by ECS. By means of immunoclectron microscopic method,the hippocampal tissue in normal and epileptic rats were taken out as the specimen and observed under a transmission electron microscope.RESULTS The hipnyampal SS irnmunoreactive (IR) neuronal perikarea and terminals following ECSshowed vacuous decrees of damages including mitochondrial swelling, breakdown of organelles and terminal degenertaion; in the multiform cell layer and molecular layer of hippocampal CA4 area, the SS-IR perikarya andterminals formed asymmetric axo-dendritic and axo-axonic synapses with non-SS - IR perikarya and terminals, and the mutual synapses were also observed betWeen terminals.CONCLUSIONS The findings suggest that the hippocampal SS neurons in the rats are damaged followingECS.