胰岛素样生长因子-Ⅰ对卵母细胞成熟度、受精力及卵裂力的影响

Experimental Study on the Effect of Insulin like Growth Factor Ⅰon Maturation Fertilization and Cleavage of Murine Oocytes in Vitro

目的：探讨胰岛素样生长因子－Ⅰ（ＩＧＦ－Ⅰ）对卵巢功能的调节作用。方法：体外培养小白鼠未成熟卵母细胞、成熟卵母细胞和精子及受精卵。实验组加入不同浓度的ＩＧＦ－Ⅰ，对照组不加ＩＧＦ－Ⅰ。观察卵母细胞成熟度、受精率及卵裂率。结果：实验组小白鼠卵母细胞成熟度、受精率及卵裂率均大于对照组，差异有极显著性及显著性（Ｐ＜０．０１及Ｐ＜０．０５）。实验组及对照组受精卵均有不均匀分裂和细胞碎片出现，但在同一培养时间内，差异无显著性（Ｐ＞０．０５）。结论：ＩＧＦ－Ⅰ可促进体外培养的小白鼠卵母细胞成熟度及受精卵的卵裂过程，提高受精率；对受精卵和胚胎无明显不良影响。

Objectives: To evaluate the effects of insulin like growth factor Ⅰ (IGF Ⅰ) on the maturation, fertilization and cleavage of murine oocytes. Methods: Immature and mature murine oocytes were cultured alone or together with murine spermatozoa in vitro. Various concentrations of IGF Ⅰ were added to the media as experimental groups, and was not added as controls. Then the rates of maturation, fertilization and cleavage of murine oocytes were observed, and compared between the 2 groups. Results: The rates of maturation, fertilization and cleavage of murine oocytes in experimental groups were significantly higher than those in the controls ( P <0.01 or P <0.05)。 Unequal blastomeres and cell fragments were observed in both groups, but the differences during the same cultured period were not statistically significant ( P >0.05). Conclusions: IGF Ⅰcould induce murine oocyte maturation, promote its fertilization and cleavage in vitro, and do no harm to the fertilized oocytes and embryos.