妊娠期糖尿病患者高密度脂蛋白糖基化与妊高征发病的关系

Glycosylated High Density Lipoprotein in Diabetes Related to Pregnancy Induced Hypertension

目的：了解妊娠期糖尿病孕妇体内高密度脂蛋白（ＨＤＬ）糖化程度及受体代谢途径情况，探讨其与妊高征发病的关系。方法：用荧光法测定妊娠期糖尿病患者糖化血红蛋白，≤８．０％为组Ⅰ，共３２例、＞８．０％为组Ⅱ，共３４例，并测定正常妊娠组３６例及正常非妊娠组３２例ＨＤＬ糖化值。用酶联免疫受体法观察ＨＤＬ与培养的人胚肺成纤维细胞表面受体特异结合情况。结果：妊娠期糖尿病患者组Ⅰ、组ⅡＨＤＬ糖化值明显高于正常妊娠组和正常非妊娠组（Ｐ＜０．０１）。其细胞结合、摄入和降解糖化ＨＤＬ均较正常妊娠组和正常非妊娠组升高，使细胞内总胆固醇含量升高（Ｐ＜０．０１）。结论：妊娠期糖尿病患者ＨＤＬ糖化值含量的变化及脂质沉积，可能是妊高征小动脉痉挛的原因之一。

Objective: To investigate the glycosylation of high densily lipoprotein (glc HDL) and its specific binding to human lung fibroblasts (HLF) receptors in gestational diabetes woman and to study its relationship with pregnancy induced hypertension (PIH). Methods: The glc HDL was measured by fluorimetry and the specific binding of HDL to HLF receptors was measured with enzyme linked immunoreceptor assay in 32 gestational diabetic woman group 1, 34 group Ⅱ,36 normal pregnant (NP) and 32 normal non pregnant (NNP) women. Results: The levels of glc HDL and the intracellular total cholesterol in diabetic pregnant woman group Ⅰand Ⅱwere significantly higher than those in the NP and the NNP ( P <0.01). The cell binding, internalization and degradation of glc HDL were higher than that in the NP and the NNP. Conclusion: The change of HDL level and lipid deposition during the process may be one of the causes for arteriole spasm in PIH.