缺血缺氧新生儿海马区锥体细胞的定量研究

Morphological change and its quantitative analysis of hippocampus in hypoxicischemic newborn

目的：探讨新生儿缺血缺氧脑损害的形态学改变及其发病机理。方法：对３９例缺血缺氧新生儿尸体解剖检查病例的大脑海马区锥体细胞进行定量观察，使用ＬｅｉｔｚＴＡＳ－Ｐｌｕｓ图像分析仪测量海马ｈ１和ｈ３段锥体细胞的数密度（Ｎｖ）和形状因子（Ｆ）。结果：测量值分别为０．９７×１０５±０．８４７ｍｍ－３（ｈ１段锥体细胞Ｎｖ），１．０７×１０５±０．７９１ｍｍ－３（ｈ３段锥体细胞Ｎｖ），０．５１１±０．０９７（Ｆ）。与对照组比较差异有显著性（Ｐ＜０．０１）。缺血缺氧新生儿海马区锥体细胞的数密度低于对照组，而形状因子大于对照组。

Purpose To study the morphological change of hippocampus and its mechanism in hypoxicischemic newborn. Methods Histologic sections of 39 autopsied cases of hypoxicischemic encephalopathy were analysed with Leitz TASPlus image analyser on which pyramidal cell numerical density (Nv) and equivalent form factor (F) of hippocampus h1 part and h3 part were determined. Results The values determined in this study were 097×105±0847 mm-3 (Nv of h1), 107×105±0791 mm-3 (Nv of h3), 0511±0097 (F). These parameters of hypoxicischemic group were significantly different from those of the contrast(P<001). Nv of hippocampus pyramidal cell in hypoxicischemic newborn was lower and F value was larger than that of the contrast. Conclusions It is considered that lesion found in the hippocampus constitute one of the morphologic bases of hypoxicischemic encephalopathy.