银杏叶制剂对脑缺血实验治疗的研究

Therapeutic Study of Folium Ginkgo Pharmaceutics on Cerebral Ischemia

采用沙土鼠短暂性脑缺血和大鼠局部脑缺血模型，缺血前３０ｍｉｎ按相当于银杏苦内酯２０ｍｇ／ｋｇ，ｉＰ给药，以观察其对缺血性脑损伤的效应．结果表明：（１）在沙土鼠双侧颈总动脉结扎１０ｍｉｎ再灌４ｄ模型，与对照组相比，银杏叶制剂明显增加海马ＣＡ１区神经元存活数，分别为３８．５０±１６．３１／ｍｍ和１３３．１３１±２０．９９／ｍｍ（Ｐ＜０．０１），与给苯妥英钠组（１６５．４２±２９．６３／ｍｍ）和Ｎｉｍｏｄｉｐｉｎｅ组（１８２．００±６７．００／ｍｍ）结果相近（Ｐ＞０．０５）．（２）大鼠左侧大脑中动脉（ＭＣＡ）凝闭后４８ｈ，与对照组相比，银杏叶制剂可使：①梗塞面积明显减小，分别为（４２．８４±１０．６１）％和（２３．０７±６．１５）％（Ｐ＜０．０５）；②梗塞中心区和周边区水含量明显降低，对照组为（８３．０３±１．１４）％，（８１．１８±１．４４）％，给银杏叶制剂组为（８１．８３±１．１３）％，（７９．６９±０．５２）％（Ｐ＜０．０５）；③梗塞中心区和周边区钠含量明显降低，对照组为（４７８．５９±８５．６５）、（４１５．６５±１５４．９３）μｍｏｌ／ｇ，给银杏叶制剂组为（３５２．４０±６３．６２），（２４３．６５±５?

Applied were the models of transient cerebral ischemia in gerbils and of regional cerebral ischemia in rats, which were administered 30 minutes before ischemia with 20 mg/kg of folium ginkgo pharmaceutics (FGP) to observe its effect on ischemic brain injury. The results were demonstrated as follows: (1) in models of gerbils, which were given 4 day perfusion 10 min after the ligation of their bilateral arteries carotid, FGP obviously increased the survival neuron number of hippocampal CA1 area (133 13±20 99/mm, P<0 01) as compared with that of control group (38 50±16 31/mm), the results being similar (P>0 05) to those of Aniline group (165 42±29 63/mm) and Nimodipine group (182 00±67 00/mm); 2) 48 hours after the arterial occlusion on the left side of rat brain, FGP could evidently decrease (a) the infarcted area (respectively 42 84±10 61% and 23 06±6 15%, P<0 05), (b) the water content at the central and peripheral areas of infarction, with (81 83±1 13)%, (81 18±1 44)% for the control group and (81 83±1 13)%, (79 69±0 52)% for FGP group (P<0 05); (c) the sodium content at the central and peripheral areas of infarction, with (478 59±85 65) μmol/g, (415 65±154 93) μmol/g for the control group and (352 40±63 62) μmol/g, (243 65±56 15) μmol/g for FGP group (P<0 05); and the calcium content at the central area of infarction, with (515 99±140 27) μg/g for the control group and (364 41±122 81) μg/g for FGP group (P<0 05). All this suggested that FGP could alleviate ischemic brain injury, lessen infarction area and palliate encephaledema.