云南汉族D_1S_(80)位点基因频率分布调查

Amp FLP Distribution Investigation of D 1S 80 Loci among Han Nationality in Yunnan

采用ＰＣＲ技术、小型聚丙烯酰胺凝胶垂直电泳银染检测法，对１６９例云南汉族进行了Ｄ１Ｓ８０位点的ＶＮＴＲ扩增片段长度多态性（Ａｍｐ－ＦＬＰ）分析，发现了１８个等位基因，５１种基因型，其分布符合Ｈａｒｄｙ－Ｗｅｎｂｅｒｇ定律．片断大小分布于４００～７５０ｂｐ之间，基因频率为０．００３０～０．２６３３．杂合度为８７％，个人识别能力为０．９５８６，对９个家系的相关个体分析符合孟德尔定律．

PCR technique and vertical eletrophoretic argentation test were applied in the Amp FLP analysis of D 1S 80 among 169 Han people in Yunnan. Discovered were 18 allels and 51 genotypes, whose distribution was in line with Hardy Wenberg law. The amplified fragments were distributed over 400～750 bp and the allele frequencies were 0 003 0～0 263 3. The heterozgosity was 87% and discrimination power was 0 958 6. The corelating individual analysis of 9 families tallied with Mengdel law.