灰葡萄孢BC7-3菌株除草活性组分的纯化与结构鉴定

Purification and structural identification of herbicides from Botrytis cinerea

【目的】植物病原真菌毒素是一类重要的微生物源除草剂,本研究旨在找到一个新的具有除草活性的化合物结构。【方法】在前期薄层层析法、柱层析法和高效液相色谱法分析的基础上对灰葡萄孢诱变菌株BC7–3的代谢产物中具有除草活性的5个不同组分分别进行了液相色谱制备。【结果】本研究得到了一个纯度达99.38%对单子叶杂草马唐具有较强杀除活性的纯组分,通过对纯组分的物理性状测定并结合紫外–可见吸收光谱、红外光谱以及核磁共振波谱等分析方法鉴定化学结构为10–顺–二氢化灰霉二醛。【结论】研究的结果为微生物除草剂的创新和开发奠定了理论基础。

[Objective] Toxin produced by phytopathogenic fungi is one of the important microbial herbicides. We found a new compound with herbicidal activity. [Methods] Five different ultraviolet absorption components were isolated from the filtrate of Botrytis cinerea isolate 7-3 culture. [Results] Of the five components, one showed strong inhibitory to Digitaria sanguinalis. The pure fraction with high herbicidal activity was obtained by HPLC purification. Its structure was identified as lO--syn-dihydrobotrydial by Ultraviolet and Visible Spectroscopy, Infrared Spectrum, Nuclear Magnetic Resonance Spectroscopy analysis. [Conclusion] The findings are important for future preparation and application of the herbicide.