云南省遗传性大肠癌组织库的建立及管理

Establishment and management of hereditary colorectal cancer tissue bank in Yunnan province

目的:为抢救和保存云南省民族性遗传性大肠癌家系标本资源,建立云南省遗传性大肠癌标本库.方法:采集遗传性大肠癌家系中患者及其直系亲属的全血、血清、血浆和粪便标本等,从中提取DNA保存.手术患者采集离体后组织标本(包括肿瘤、瘤旁和远端正常组织)、石蜡包埋切片.同时采集散发性大肠癌全血和组织标本提取DNA作为对照.并开发一套信息管理系统应用于标本库的管理,系统运行平台为WindowXP,数据库为ACCESS2003.结果:2007-09/2008-06共采集14个遗传性大肠癌家系(10个FAP家系,4个HNPCC家系),其中包括9个汉族家系,2个白族家系,2个彝族家系,1个藏族家系的标本.其中血液标本达356份,组织标本157份,提取所有家系成员的DNA备用.并同时采集散发性大肠癌血液样本354份其中非汉民族127份.结论:建立了规范的,具有一定规模的云南省民族性遗传性大肠癌标本库.

AIM： To establish a tissue bank of hereditary colorectal cancer to rescue and preserve the national resources of hereditary colorectal cancer family in Yunnan province. METHODS： Specimens of whole blood, serum, plasma and stool of patients and their lineal con-sanguinity in hereditary colorectal cancer fam-ily were collected and then DNA was extracted from these specimens for preservation. Tissue specimens in vitro （including tumor tissues, peri-tumor tissues and normal tissues） were collected and paraffin-embedded sections were prepared of operative patients. At the same time, DNA specimens of whole blood and tumor tissue of sporadic colorectal cancers were taken as con-trols. Simultaneously we developed an informa-tion management system for tissue bank with the operating system platform for Window XP, database ACCESS2003. RESULTS： From September 2007 to June 2008, we collected 14 hereditary colorectal families （10 FAP families and 4 HNPCC families） including 9 Han families, 2 Bai families, 2 Yi families and 1 Zang family. There were 356 blood specimens and 157 tissue specimens. DNA of all family members was extracted in reserve. And 354 blood specimens of sporadic colorectal cancer including 127 ones of non-Han nationalities were collected. CONCLUSION： We established a standard tissue bank of hereditary colorectal cancer in Yun-nan province.