Survivin和APC蛋白在原发性胆囊癌中的表达及意义

Expressions of Survivin and APC Protein in Primary Gallbladder Carcinoma and Significance

目的:研究survivin和APC蛋白在原发性胆囊癌中的表达情况,并分析其与病理特征可能关系。方法:收集胆囊癌标本50例、胆囊腺瘤20例、慢性胆囊炎20例。应用免疫组织化学方法检测survivin蛋白和APC蛋白的表达情况,同时收集胆囊癌患者的病理资料,并将结果进行分析。结果:(1)survivin蛋白在胆囊癌、胆囊腺瘤及慢性胆囊炎组织中阳性表达率分别为64%(32/50)、20%(4/20)和10%(2/20),差异有显著性(P<0.05)。胆囊癌组与胆囊腺瘤组及胆囊炎组比较差异均具有显著性(P<0.05)。Sur-vivin阳性表达与病理资料均无明显相关性(P>0.05)。(2)APC蛋白在胆囊癌、胆囊腺瘤及慢性胆囊炎组的阳性表达率分别为48%(24/50)、85%(17/20)及90%(18/20),差异有显著性(P<0.05)。胆囊癌组阳性表达率显著低于胆囊腺瘤组和慢性胆囊炎组(P<0.05)。APC蛋白的表达水平与患者的病理资料均无明显相关性(P>0.05)。(3)Survivin阳性表达与APC蛋白表达呈明显负相关(rs=-0.530,P=0.000)。结论:胆囊癌组survivin蛋白的表达显著高于胆囊腺瘤及胆囊炎组,说明凋亡可能在胆囊癌的发生中起重要作用;survivin和APC蛋白在胆囊癌中的表达呈明显的相关性,可能在胆囊癌的发生中起协同作用。

Objective：To investigate expressions of survivin and APC protein in gallbladder carcinoma and analyze its possible relation with pathological characters. Methods： Expressions of survivin and APC were determined by an immunohistochemistrical method in 50 cases with carcinoma of gallbladder, 20 with adenoma of gallbladder and 20 with chronic＇ cholecvstitis. The pathological data from patients with earcinoma of gallbladder was analyzed. Results：The positive rates of survivin and APC protein in carcinoma of gallbladder, adenoma of gallbladder and chronic cholecystitis were 64.0% （ 32/50 ） and 48.0% （ 24/50 ）, 20.0% （4/20） and 85.0% （ 17/ 20） , 10.0% （2/20） and 90.0% （18/20） , respectively. The positive expression of survivin in carcinoma of gallbladder was significantly higher than those in adenoma of gallbladder and cholecystitis （ P 〈 0.05 ） , however, the corresponding expression of APC protein was significantly lower than those in adenoma of gallbladder and eholecystitis （ P 〈 0.05 ）. Both expressions of survivin and APC protein in gallbladder carcinoma had no significant correlation with pathological features（ P 〉 0.05 ）. Based on this findings, we could see the expression of survivin in carcinoma of gallbladder was negative correlation with expression of APC protein （ rs = -0. 530,P = 0. 000）. Conclusion：The results, i.e. the positive rates of survivin in gallbladder carcinoma were significantly higher than those in gallbladder adenoma and cholecystitis, illustrate that apoptosis may play an important role in the carcinogenesis. Both expressions of survivin and APC protein in gallbladder carcinoma have significant correlation, suggesting they may play a synergistic effect in the carcinogenesis of gallbladder carcinoma.