非酒精性脂肪性肝病大鼠肝脏脂联素受体的表达

Expression of adiponectin receptor of liver in rats with nonalcoholic fatty liver disease

目的探讨非酒精性脂肪性肝病(NAFLD)大鼠肝脏脂联素受体(AdipoR)mRNA的表达。方法喂养高脂饲料建立NAFLD大鼠模型,分别于2、4、8、12周检测血清生化指标,并取肝组织测肝指数(肝湿重/体重),以RT-PCR法检测肝脏AdipoR1和AdipoR2 mRNA的表达,肝组织冰冻切片苏丹Ⅲ脂肪染色、石蜡切片苏木素-伊红常规染色和Masson三色纤维染色,镜下观察。结果模型组大鼠2、4、8、12周肝脏AdipoR1 mRNA表达逐步上升,AdipoR2 mRNA表达逐步下降,两者分别于4周、2周开始显著差异于对照组(P均<0.01)。模型组肝脏AdipoR2表达与肝指数(r=-0.431,P=0.006)、纤维化评分(r=-0.353,P=0.025)均呈显著负相关。结论NAFLD大鼠肝脏AdipoR1 mRNA表达增加,AdipoR2 mRNA表达减少,提示肝脏AdipoR表达异常可能参与NAFLD的发病机制。

Objective To investigate the expression of hepatic adiponectin receptor in the rats with nonalcoholic fatty liver diseases（NAFLD）. Methods The NAFLD model rats were induced by high fat diet. The rats were killed at 2, 4, 8, 12 weeks respectively. The serum biochemical parameters were measured, liver index evaluated and hepatic AdipoR1 mRNA and AdipoR2 mRNA expressions measured by RT-PCR. Liver slices were analyzed for pathological changes. Results In the model group, the expression of hepatic AdipoR1 mRNA were gradually increased, the AdipoR2 mRNA expression decreased as compared with the control group （P 〈 0. 01 ）. AdipoR2 expression was negatively correlated with liver index （ r = -0. 431, P = 0. 006） and hepatic fibrosis scale （ r = - 0. 353, P =0. 025 ） with statistical significance, respectively. Conclusion Hepatic AdipoR1 mRNA expression were increased and the AdipoR2 mRNA expression decreased in NAFLD rats. The results indicate that abnormal hepatic AdipoR expression may be related to the pathogenesis of NAFLD.