芪倍液体外抗Ⅱ型单纯疱疹病毒的实验研究

Experimental Study on Liq Qibei against Herpes Simplex Virus-Ⅱ in Vitro

目的研究芪倍液体外抗Ⅱ型单纯疱疹病毒(HSV-Ⅱ)的作用机理。方法利用体外细胞培养技术,采用微量细胞病变抑制法来研究中药各浓度组的抑制病毒增殖作用、直接杀伤病毒作用,以病毒滴度和抑制病毒指数为指标。结果①抑制病毒增殖实验:芪倍液1.63、0.81、0.41mg/mL浓度组的病毒滴度与病毒对照组比较,差异有统计学意义(P<0.01),抑病毒指数>2;②直接杀伤病毒实验:芪倍液1.63mg/mL-8h组、1.63mg/mL-24h组、0.81mg/mL-24h组的病毒滴度与病毒对照组比较,差异有统计学意义(P<0.05),其余各组的病毒滴度与病毒对照组比较,差异无统计学意义(P>0.05)。结论芪倍液具有抗Ⅱ型单纯疱疹病毒的作用。

Objective To explore the effect and mechanism of Liq Qibei against herpes simplex virus-Ⅱ （HSV-Ⅱ） for treatment of cervical erosion. Methods Using micro-inhibition cytopathic and in vitro ceU culture technique, the various concentrations of Liq Qibei in inhibiting HSV-Ⅱ proliferation and the direct virucidal effect were studied. The virus titer and virus inhibition index were taken as parameters. Results （1） Virus proliferation experiment： Compared with control group, the virus titer of 1.63, 0.81, 0.41 mg/mL Liq Qibei group had significant difference （P〈O.01）, the virus inhibiting index 〉2. （2） Direct virucidal effect of Liq Qibei： comparing with control group, 1.63 mg/mL Liq Qibei in 8 h, 24 h and 0.81 mg/mL-24 h groups had marked difference （P〈0.05）. The virus titer of other groups were not significantly different from the control group （P〉0.05）. Conclusion Liq Qibei antivirus effect on HSV- Ⅱ.