威斯康星卡片分类测验作为双生子基因功能稳定性指标的初探

A Pilot Study of Wisconsin Card Sorting Test Parameters as the Lndex Stability of Twins Gene Function

目的:探索威斯康星卡片分类测验测试各指标中更稳定、更精确的指标(内表型)。方法:在重庆市主城区募集6-16岁的双生子。签写知情同意书后,用威斯康星卡片分类测验对59对6-16岁的双生子测试(同卵双生28对,异卵双生31对),比较双生子两个体之间的六个常用指标(正确数、错误数、持续错误数、非持续错误数、总分类数、完成第一个分类所需个数)得分的相关系数,采取双生子的颊黏膜标本以提取DNA并进行卵型鉴定。结果:MZ组和DZ组的年龄、性别、受教育年限具有可比性(P>0.05),MZ组的持续错误数指标在双生子对个体之间的相关系数有显著相关(r=0.65,P=0.001),其余五个指标和DZ组的六个指标在双生子对个体之间的得分无显著性相关(P>0.05)。结论:在威斯康星卡片分类测验常用的六个指标中,持续错误数受遗传的影响更大,作为内表型指标可能优于威斯康星卡片分类测验中的其他指标。

Objective： To explore the more stable and precise index （ endophenotype） in the Wisconsin Card Sorting Test （ WCST） . Methods： Twins aged 6 to 16 who lived in Chongqing were recruited. After guardians of twins had signed the comprehending and consent document, the Wisconsin Card Sorting Test （ WCST） was applied to 59 twin pairs, 28 pairs monozygotic twins （ MZ ） and 31 pairs dizygotic twins （ DZ ） . The intrapair correlation coefficients of twins were compared. Buccal mucosa samples which DNA were extracted from all twins. Zygosity identification test were applied to all samples. Results： The intrapair correlation coefficient of preservative errors in Monozygotic twins was more remarkable than that in dizygotic twins among 6 commonly used indexes in WCST （ r =0. 65, P =0. 001 ） Conclusion： Among the six commonly used indexes of WCST, the preservative errors are influenced mostly by heredity and are more reliable than the rest, which could be used as distinguished endophenotype.