莫迦小麦(Triticu mmacha L.)T型恢复基因Rf3和K型不育基因rfv1的连锁关系

Linkage Relationship between T-type Restorer Gene Rf_3 and K-type Male Sterile Gene rfv_1 in Triticum macha L.

为进一步明确莫迦小麦(Triticum macha)T型恢复基因Rf3与K型不育基因rfv1的连锁关系,利用T型细胞质背景(T504A/Tm3314F2代和T504A//KTm3314A/90(13)21杂交分离群体)的可育株在K型细胞质下的育性测交分析,明确了来自莫迦小麦的这2个基因连锁并不紧密,交换值约为16.54%。可利用T型主效恢复基因Rf3提高含有T型主效恢复基因和K型主效不育基因的基础材料的选择效率。

Wheat （Triticum aestivum L.） breeding line Tm3314 is a T-type non-1B/1R material carrying 1BS of T. macha. KTm3314A, a K-type non-1B/1R male sterile line developed from Tm3314, is easily restored in fertility, does not produce hap- loids in reproduction, and has high temperature-sensitivity, thus has potential in wheat hybrid breeding. The T-type restorer gene Rf3, which is closely linked to K-type male sterile gene rfv1 on IBS in T. spelta, is used as a marker of rfkl under the background of T. timophvee cytoplasm. However, some male sterile materials with substitutive chromosome fragments of the 1BS of T. macha have been found the absence of rfv1 under T. timophvee cytoplasm background. To understand the linkage relationship between Rf3 and Ffv1 in T. macha, the segregation generations of （T504A/Tm3314） F2 and T504A//KTm3314A/90（13）21 as well as their backcross populations with a K-type sterile line K119A were used in field experiments in 2003-2006. The results showed that cross- ing-over value between Rf3 and rfv1 was 16.54%.